Flow cytometric screening of aldolase catalytic antibodies.
نویسندگان
چکیده
High-throughput screening of cells expressing active catalytic antibody clones by flow cytometry is described. A fluorogenic retro-aldol retro-Michael substrate was designed and synthesized with incorporation of a chloromethyl moiety for intracellular retention. Hybridoma or transfected mammalian cells expressing catalytic antibody molecules could be rapidly isolated.
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 14 15 شماره
صفحات -
تاریخ انتشار 2004